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Advanced Search Abstract The mdx mouse model of muscular dystrophy arose due to a nonsense mutation in exon 23 of the dystrophin gene. This results in an in-frame mRNA transcript and subsequent expression of a slightly shorter dystrophin protein in mdx muscle. Refinement of AO design has allowed efficient exon skipping to be induced in mdx mouse muscle cultures at nanomolar concentrations. The morpholino chemistry produces a neutral molecule that has exceptional biological stability but poor cellular delivery. We have investigated a number of leash designs and chemistries, including mixed backbone oligonucleotides, and their ability to influence delivery and efficacy of the morpholino AO. Significantly, we detected dystrophin protein synthesis and correct sarcolemmal localisation after intramuscular injection of morpholino AO:
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Cooling the body is especially beneficial to the elderly, and this research confirms previous studies that found warmer skin, in both humans and animals, disrupts sleep. Staying cool through the night has also been found to increase your metabolism. This good fat will help you burn calories while it generates body heat. Skipping clothes isn't just recommended for females, but it is important for a man in order to maximize their sperm production. A lowered body temperature at night has also been found to help you burn calories while you sleep. For women, it helps prevent bacterial infections such as yeast infections. And it increases a man's sperm production by keeping his scrotum at a specific temperature.
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